كيف يتم عمل تحليل عد خلايا الدم الحمراء RBCs count/ hemocytometer/Procedure and Calculations

In this video, we’re gonna count the number of red blood cells in a sample of human/animal blood using the haemocytometer. Requirements: Counting chamber RBC pipette Blood sample RBC diluting solution Sodium Chloride 1.0g (provides isotonic environment) DCompound light microscope Cotton and Rubbing alcohol Take the RBC pipette precleaned with alcohol. Draw the blood sample till the 0.5 mark using your mouth or a syringe, while taking care to avoid any air bubbles from entering the pipette. This step requires a bit of patience and practice. If you happen to suck the blood beyond the 0.5 mark, you can either use a clean filter paper to carefully blot out excess blood from the pipette tip, or carefully blow out excess blood using a syringe until it reaches down to the 0.5 mark. Once that’s done, carefully clean the excess blood sticking to the outer sides of the tip of the pipette using a cotton soaked in alcohol. Now carefully draw the RBC upto 101 mark on the pipette. Hold the pipette in a horizontal position and Rotate the pipette several times in such a way that the tiny bead inside the bulb of the pipette mixes the blood and the diluting solution thoroughly. Once thorough mixing has been accomplished, the blood sample now has a 200 times dilution. Once this is done, prepare the counting chamber for sample loading. Take the counting chamber and the cover slip, both precleaned with alcohol. Carefully position the coverglass on top of the support platforms located on both sides of the counting chamber platforms. Discard the first few drops from the RBC pipette. Carefully position the tip of the pipette on one edge of the coverglass. Allow the contents of the pipette to gradually flow into the narrow space between the coverglass and the counting chamber via a capillary force like you see here. You may load the other counting chamber as well in a similar fashion. Once ur done loading the chambers, keep aside the counting chamber for a few minutes to allow the RBCs to settle. The slide is now ready for microscopic observation. First locate the small square at the centre of the counting grid under low power of the microscope. First locate the large squares at the four corners of the counting grid under low power of the microscope. You may refer to my previous video on Haemocytometer to see which of the squares in the counting chamber are to be used for RBC counting. You may also need to adjust the light intensity of the microscope in order to visualise the fine lines in the counting grid. Once you’ve located the RBC counting squares, switch to 10x then to 40x objective to begin the counting. Locate the 1st, 5th, 13th, 21st and 25th smaller squares in the RBC grid. Applying the Margin Rule or L rule of cell counting, Count the RBCs lying inside of these squares and those lying on the borders of the lower and left sides of each square. Do not count those cells lying on the borders of the upper and right sides of the squares. Remember the margin rule and count the cells in each square accordingly. how blood count is measured, Total RBC Count Practical Lab how complete blood count is performed, what is blood count hemocytometer calculation hemocytometer calculator hemocytometer counting hemocytometer cell counting hemocytometer formula haemocytometer calculation hemocytometer calculation examples hemocytometer شرح, hemocytometer physiology, hemocytometer physiology practical, hemocytometer cell count, hemocytometer chamber, hemocytometer for cell counting, hemocytometer guide, glass hemocytometer, hemocytometer how to use, hemocytometer practical, hemocytometer test, hemocytometer tutorial, hemocytometer volume load, hemocytometer video, hemocytometer vs counting chamber bloodcount# high blood count means, blood count normal, cbc blood count normal ranges, count no of blood تجربة عد كريات الدم الحمراء عد الدم الاحمر يدويا, عد كريات الدم الحمراء, عد خلايا الدم الحمراء, عد كرات الدم الحمراء يدويا, عد كريات الدم الحمراء يدويا, عد كرات الدم الحمراء how blood count is measured what blood count is low is blood countable or uncountable blood count levels Hemocytometer # - Counting of cells blood count test blood count normal range blood count range blood count blood test r b c blood count c count blood test blood count exam what is normal blood counts how many blood count in human body what should blood count be for anemia what should blood count numbers be what blood count is considered anemic what blood count shows anemia when blood count is low when blood count is high when your blood count is low what happens when red blood count is high when blood count where should your red blood count be what will happen if blood count is low which blood count shows anemia

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